Animals were immunized with C57BL/6 thymocytes, boosted three times with the RBL-CD1.1 rat cell line (CD1.1-transfected), boosted once with BALB/cJ thymocytes and finally with the CR1-CD1.1 human cell line (CD1.1-transfected). Spleen cells were fused with Sp2/0-Ag14 cells. The antibody reacts with CD1.1, but cross-reacts with CD1.2 only at very high concentrations. The antibody blocks recognition of CD1.1 by T cells in vitro.
Application Notes
FC; IP
Morphology
Lymphoblast
Growth Properties
Mixed: Adherent and Suspension. The cells are rather Adherent in nature, and grow best if maintained in Suspension culture plates (e.g., Corning Catalog number 430591).
CultureMethod
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
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