Mouse Anti-Bovine Myosin Hybridoma [CB-G9]

We studied for the first time a collection of 377 snakes assembled by Benoit Mys and Jan Swerts in Papua New Guinea (PNG) during 1982-85. The collection, stored at the Royal Belgian Institute of Natural Sciences (RBINS), contains specimens collected in rapid assessments of 45 mainland and island localities in Madang, East Sepik, West Sepik, and Morobe Provinces, and 8 localities from West New Britain and Manus Provinces. Thirty-three species from six families (Acrochordidae, Boidae, Colubridae, Elapidae, Pythonidae, Typhlopidae) were present in the collection, and we report a number of new morphological observations from unidentified Dendrelaphis, Tropidonophis, and Aspidomorphus, as well as from Stegonotus cf. parvus. We report 31 new island records for snakes. Combined with island distributional information extracted from literature and museum collections, we provide an updated overview of snake species occurrences on the islands off the north coast of PNG. The substantial contribution of a 30-yr-old museum collection to current knowledge of the snake fauna of northern PNG illustrates how poorly studied this region is and the extent of herpetofaunal work still required to uncover true snake diversity in PNG.

Temperature and dissolved oxygen concentration are important abiotic factors that can limit the growth and survival of sea cucumbers by affecting their immune systems. As global warming intensifies, sea cucumbers are increasingly exposed to adverse environmental conditions, which can cause severe economic losses and limit the sustainable development of sea cucumber aquaculture. It is therefore important to better understand how sea cucumbers respond to environmental stress, especially with regard to its effects on immunity. In the present study, the time series of immunity-related gene expression in sea cucumbers under thermal and hypoxic stresses were analyzed separately. The expression trends of 17 genes related to the nuclear factor kappa B (NF-kappa B) pathway, the protease family, the complement system, heat shock proteins (HSPs) and the transferrin family during exposure to two stresses at eight time points were concluded. These genes have interconnected roles in stress defense. The expression levels of genes relating to the NF-kappa B pathways and HSPs were strongly affected in the sea cucumber thermal stress response, while melanotransferrin (MO), ferritin (Ft) and mannan-binding C-type lectin (MBCL) were affected by hypoxia. In contrast, complement factor B (Bf), myosin V (Mys) and serine protease inhibitor (SPI) were not that sensitive during the initial period of environmental stress. Similar expression patterns under both thermal and hypoxic stress for certain genes, including an increase in Hsp90 and decreases in lysozyme (Lys), major yolk protein (MYP) and cathepsin C (CTLC) were observed in sea cucumbers. Conversely, NF-kappa B and Hsp70 were differentially affected by the two stress treatments. Lysozyme-induced immune defense was inconstant in sea cucumbers coping with stress. A gene ontology (GO) analysis of the selected genes revealed that the most co-involved terms related to immunity and iron ion. Our analysis suggests that sea cucumbers demonstrate complex and varied immune responses to different types of stresses. This dynamic image of the immune responses and stress tolerance of sea cucumbers provides new insights into the adaptive strategies of holothurians in adverse environments.