Bovine B cells in peripheral blood and lymph nodes
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
NS1
Clone
DD67
Cell Line Description
Animals were immunized with bovine mesenteric lymph node cells. Spleen cells were fused with NS1 myeloma cells. The antibody reacts with bovine B cells in peripheral blood and lymph nodes. It precipitates a 180000 dalton molecule from the surface of bovine B cells. This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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