Animals were immunized with spleen cells from DBA/2 mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody induces blastogenesis in B cells, and this response is limited to the Lyb 5+ class of B cells. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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