GeneralInformation
Immunogen
Cells of the TK1 murine T cell lymphoma
Fusion Species
Rat X Mouse Heterohybridoma
Immunological Donor
Rat spleen
Cell Line Description
Animals were immunized with cells of the TK1 murine T cell lymphoma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the mouse lymphocyte mucosal homing receptor. It is specific for mouse alpha 4, beta 7 heterodimers, and can induce alpha 4, beta 7 dependent lymphocyte aggregation. The antibody inhibits alpha 4, beta 7 mediated adhesion activities including binding to MAdCAM-1, and alpha 4, beta 7 dependent VCAM-1 and fibronectin binding. DATK32 is useful in flow cytometry methods using sensitive second stage reagents, but is ineffective in immunohistological staining.
Application Notes
It is useful in FC methods using sensitive second stage reagents, but is ineffective in immunohistological staining.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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COA
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