Anti-AATM monoclonal antibody, clone F-8

Antibody Isotype

IgG2b

Clone

F-8

Species Reactivity

Human

Immunogen

The immunogen is amino acids 141-211 mapping within an internal region of AATM of human origin.

Conjugate

Application Notes

Western Blotting (starting dilution 1:200; dilution range 1:100-1:1000);
Immunoprecipitation [1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)];
Immunofluorescence (starting dilution 1:50; dilution range 1:50-1:500)

Antigen Description

Aspartate aminotransferase (AAT) is an ubiquitous pyridoxal phosphatedependent enzyme, which exists in both mitochondrial (AATM) and cytosolic (AATC) forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles by catalyzing the conversion of L-aspartate and 2-oxoglutarate to oxaloacetate and L-glutamate. The two isoenzymes are homodimeric, but differ in expression patterns. Approximately 80% of the enzyme activity in liver is of mitochondrial origin, whereas in serum the enzyme activity is largely cytosolic. AATC and AATM share nearly identical three-dimensional structures, but differ in their folding rates and in their affinity for binding to molecular chaperones, including GroEL.