Annexin V-PE/7-AAD Apoptosis Detection Kit

Sample

Cultured cells

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection of phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry.

Contents of Kit

Storage

2 - 8°C

General Description

Annexins are a family of calcium-dependent phospholipid-binding proteins. They are abundant in eukaryotic organisms belonging to a family of ubiquitous cytoplasmic proteins involved in signal transduction. All annexins have been shown to have a putative binding site for protein kinase C (PKC) but only annexin V would possess a potential pseudosubstrate site. Thus, annexin V seems to modulate the activity of some PKCs on their substrates. Annexin V was found to play a major role in matrix vesicle-initiated cartilage calcification as a collage-regulated calcium channel. Annexin V binds to procoagulant phospholipids (Vascular anticoagulant alpha) with high affinity. Annexin V's preferential binding partner is phosphatidylserine (PS). PS is predominantly located in membrane leaflets, which face the cytosol. However, recent findings show that each cell type has the molecular machinery to expose PS at its cell surface. This machinery is activated during the execution of apoptosis. Once PS is exposed at the cell surface it exhibits procoagulant and proinflammatory activities. Annexin V will bind to the PS-exposing apoptotic cell and can inhibit the procoagulant and proinflammatory activities of the dying cell.

Reconstitution And Storage

The kit is shipped on blue ice. Storage at 2 - 8°C is recommended.