Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Acetylcholinesterase (AChE) activity in serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 20 mL x 1 | 4°C |
| Substrate | Powder x 1 | 4°C, keep in dark |
| Dye Reagent | Powder x 1 | 4°C, keep in dark |
| Standard | Powder x 1 | 4°C |
| Positive Control | Powder x 1 | -20°C |
| Instruction Manual | 1 | |
Detection Range
0.1 mmol/L - 5 mmol/L
General Description
Acetylcholinesterases (AChEs) are enzymes that hydrolyze the neurotransmitter acetylcholine (ACh) to and choline. AChE is located at the synaptic cleft and functions to terminate synaptic transmission by catalyzing the breakdown of ACh allowing cholinergic neurons to return to a resting state after activation. Changes in AChE activity may result from exposure to certain insecticides, which act as cholinesterase inhibitors. Inhibitors of AChE are also used to treat certain conditions such as dementia. The Acetylcholinesterase Activity Microplate Assay Kit provides a simple and direct procedure for measuring AChE levels in a variety of samples such as blood, serum, and plasma. In this assay, thiocholine produced by AChE, reacts with DTNB to form a colorimetric (412 nm) product (TNB), proportional to the AChE activity present.
Citations
Publication ()
Have you cited DFLA26068 in a publication?
Let us know and earn a reward for your research.
COA
Loading ......
