Sample
Serum, plasma, tissue homogenates
Intended Use
For the quantitative determination of fish acetylcholinesterase (AChE) concentrations in serum, plasma, tissue homogenates.
Storage
The unopened kit should be stored at 2 - 8°C. Do not use the kit beyond the expiration date.
Opened kit may be stored for up to 1 month at 2 - 8°C.
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Detection Range
2.5 ng/ml-40 ng/ml.
Sensitivity
The minimum detectable dose of fish AChE is typically less than 2.5 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest fish AChE concentration that could be differentiated from zero.
General Description
Acetylcholinesterase, also known as AChE or acetylhydrolase, is a serine protease that hydrolyzes the neurotransmitter acetylcholine. AChE is found at mainly neuromuscular junctions and cholinergic brain synapses, where its activity serves to terminate synaptic transmission. It belongs to carboxylesterase family of enzymes.
Citations
Publication ()
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