Pig coagulation factor X ELISA kit

Sample

serum, plasma, tissue homogenates

Species Reactivity

Porcine

Intended Use

For the quantitative determination of pig coagulation factor X (F10) concentrations in serum, plasma, tissue homogenates.

Storage

1. The unopened kit should be store at 2 - 8°C. Do not use the kit beyond the expiration date.
2. Coated assay plate may be stored for up to 1 month at 2 - 8°C. Try to keep it in a sealed aluminum foil bag, and avoid the damp.
3. Standard and HRP-conjugate may be stored for up to 1 month at 2 - 8° C. If don't make recent use, better keep it store at -20°C.
4. The other reagents may be stored for up to 1 month at 2 - 8°C.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

0.312 μg/mL-20 μg/mL

Sensitivity

The minimum detectable dose of pig F10 is typically less than 0.027 μg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.