Mouse Anti-Human F10 Light Chain Monoclonal antibody, clone HU379

Bioactivity-guided separation led to the isolation of six novel phenanthrenes, spiranthesphenanthrenes A-F (1-6), together with 19 known compounds, including seven phenanthrenes (7-13), one bibenzyl compound (14), five flavonoids (15-16 and 20-22), and six simple phenolic compounds (17-19 and 23-25), from the petroleum ether (PE) and ethyl acetate (EtOAc) extracts of Spiranthes sinensis (Pers.) Ames, an edible medicinal plant named "panlongshen" in Chinese that is popularly used in medicinal foods and herbal teas. The structures of the obtained compounds were identified on the basis of extensive NMR spectroscopy and HR-ESI-MS analyses. The cytotoxicities of the phenanthrenes (1-13), the bibenzyl compound (14), and the flavonoids (15-16 and 20-22) toward SGC-7901, HepG2, and B16-F10 cell lines were examined in vitro. Compounds 1 and 7 exhibited moderate cytotoxic activities toward all of the selected cancer cell lines, and their IC50 values ranged from 19.0 +/- 7.3 to 30.2 +/- 5.6 mu M. Spiranthesphenanthrene A (1) exhibited higher cytotoxic activity than the positive control cisplatin toward the B16-F10 cell line (IC50 = 19.0 +/- 7.3 mu M). A wound healing assay revealed the inhibition of the migration of B16-F10 cancer cells in a time- and dose-dependent pattern by treatment with 2.5, 5, and 10 mu M solutions of compound 1 for 24 and 48 h, respectively. Western blots revealed that compound 1 obviously increased the level of the E-cadherin protein (an epithelial marker) and decreased the levels of the vimentin and N-cadherin proteins (mesenchymal markers). Furthermore, the level of the transcription factor Snail was also obviously decreased by compound 1 in a dose-dependent manner. Taken together, compound 1 inhibits the migration of B16-F10 cancer cells, which may be closely related to the inhibition of the epithelial-mesenchymal transition. Compound 1 represents a promising drug candidate for the prevention of tumor metastasis.

The Dual-zone Aperture X-ray Solar Spectrometer (DAXSS) was flown on 2018 June 18 on the NASA 36.336 sounding rocket flight and obtained the highest resolution to date for solar soft X-ray (SXR) spectra over a broad energy range. This observation was during a time with quiescent (nonflaring) small active regions on the solar disk and when the 10.7 cm radio flux (F10.7) was 75 solar flux units (1 sfu = 10-22 W m(-2) Hz(-1)). The DAXSS instrument consists of a LASP-developed dual-zone aperture and a commercial X-ray spectrometer from Amptek that measures solar full-disk irradiance from 0.5 to 20 keV with a resolving power of 20 near 1 keV. This paper discusses the novel design of the spectrometer and the instrument characterization techniques. Additionally, the solar measurements obtained from the 2018 sounding rocket flight are analyzed using CHIANTI spectral models to fit the temperatures, emission measures, and relative elemental abundances of the solar corona plasma. The abundance of iron was found to be 35% higher than expected in the quiescent Sun's corona suggesting either that our spectral models require additional sophistication or that the underlying atomic database may require updates. Future long-term systematic observations of this spectral range are needed to provide further insight into the sources of coronal heating through modeling the changes of relative elemental abundances during developments of active regions and solar flaring events.