One unit is defined as the amount of enzyme which converts 1 μ mole of 3‒Hydroxybutylate to acetoacetate per minute at 37°C under the conditions specified in the assay procedure.
Target
3-Hydroxybutyrate Dehydrogenase
Molecular Weight
30 kDa
Application Notes
This enzyme is useful for enzymatic determination of ketone bodies when coupled with acetoacetate decarboxylase(AADC), thio‒NAD and NADH
Format
Lyophilized
Buffer
Enzyme dilution buffer
Storage
Store at -20°C
Introduction
3-hydroxybutyrate dehydrogenase has a classification number of EC 1.1.1.30. The first digit means that this enzyme is an oxidoreductase which means the purpose is to catalyze oxidation and reduction reaction pathways. The following two 1s indicate the subclass and sub-sub of the enzyme. In this case, 1.1.1 means this enzyme is an oxidoreductase that acts on the CH-OH group of the donor molecule using NAD(+) or NADP(+) as the acceptor.[2] The 4th number, or 30 in this case, is the serial number of the enzyme to define it within its sub-subclass.
Keywords
3-Hydroxybutyrate Dehydrogenase; BHBDH
Citations
Publication ()
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