mRNA (nucleoside-2'-O-)-methyltransferase ELISA Kit

Name: mRNA (nucleoside-2'-O-)-methyltransferase ELISA Kit
SKU: DEIA-BZ001
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Biological products.

Detection Method

Sandwich-ELISA

Intended Use

This assay kit is capable for the quantitative determination mRNA (nucleoside-2'-O-)-methyltransferase.

Contents of Kit

No.	Components	Size	Storage Conditions
1	2'-O-Methyltransferase standard	100 μL	-20°C
2	Microtiter strips	96T	2-8°C
3	Sample diluent	12 mL	2-8°C
4	ELISA detection antibody diluent	12 mL	2-8°C
5	Enzyme-labeled detection antibody (100×)	120 μL	2-8°C
6	Washing solution (20×)	30 mL	2-8°C
7	Substrate solution	12 mL	2-8°C
8	Stop solution	6 mL	2-8°C
9	Parafilm	3 sheets

Storage

BOX 1, store at -30—-15°C; BOX 2, store at 2—8°C.
Do not freeze kits and store away from light. The expiry date is specified on outer labels.

Precision

Sensitivity

LOD: 8.15 pg/mL, LOQ: 125 pg/mL

General Description

mRNA (nucleoside-2'-O-)-methyltransferase is a methyltransferase encoded by the DNA of the vaccinia virus. This enzyme uses S-Adenosylmethionine (SAM) as a methyl donor to add methyl groups to the 2'-O site of the first nucleotide at the 5' end of RNA adjacent to the cap structure to form mRNA with Cap1 structure. Cap1 structure can enhance the efficiency of mRNA translation and reduce the immunogenicity of mRNA structure itself, thus helping to improve the expression level of encoded protein after mRNA transfection. mRNA (nucleoside-2'-O-)-methyltransferase can specifically recognize the m7GpppN (cap0) structure but does not act on RNA with the 5' end pN, ppN, pppN or GpppN.

Standard Curve