Mouse Rln3 (Relaxin-3) ELISA Kit

Name: Mouse Rln3 (Relaxin-3) ELISA Kit
SKU: DEIA-FN1272
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma, cell culture supernatants, tissue homogenate

Species Reactivity

Mouse

Detection Method

Sandwich ELISA

Intended Use

For quantitative detection of Mouse Rln3 (Relaxin-3) in serum, plasma, tissue homogenates and other biological fluids.

Contents of Kit

1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Detection Range

1.56-100 mIU/mL

Sensitivity

0.938 mIU/mL

Standard Curve

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Distribution of Relaxin-3 mRNA and Immunoreactivity and RXFP3-Binding Sites in the Brain of the Macaque, Macaca fascicularis

RELAXIN AND RELATED PEPTIDES: FIFTH INTERNATIONAL CONFERENCE

Authors: Ma, Sherie; Shen, Pei-Juan; Sang, Qian; Lanciego, Jose L.; Gundlach, Andrew L.

Abstract

This study examined the presence and distribution of relaxin-3 (RLN3) and its receptor, RXFP3, throughout the brain of Macaca fascicularis. In perfusion-fixed sections, RLN3 immunoreactivity (IR) was observed throughout the fore-, mid-, and hindbrain and was enriched in the septum, hippocampus, and interpeduncular and supramammillary nuclei. Neurons in the pontine central gray area contained RLN3-IR and RLN3 mRNA. The distribution of RXFP3 protein, mapped by autoradiography of [I-125]R3/I5-binding sites, correlated with the distribution of RLN3-IR. The broad distributions of RLN3-positive neuronal axons and terminals and of RXFP3 in the brain of Macaca fascicularis are consistent with findings in the rat and with an important role for RLN3 signaling in nonhuman primate and human brain.

Polymorphisms in the Promoter Region of Relaxin-2 and Preterm Birth: Involvement of Relaxin in the Etiology of Preterm Birth

IN VIVO

Authors: Vogel, Ida; Hollegaard, Mads Vilhelm; Hougaard, David Michael; Thorsen, Poul; Grove, Jakob

Abstract

Background: Circulating relaxin levels have repeatedly been associated with preterm birth (PTB). Our aim was to investigate if mothers carrying promoter single nucleotide polymorphisms (SNPs) in one of the three relaxin genes (RLN1, RLN2, RLN3) are predisposed for PTB. Patients and Methods: Maternal DNA from 80 preterm cases (40 very preterm births (24-34 weeks) and 40 moderate preterm (34-36 weeks) and 40 controls (term delivery)) nested in the Danish National Birth Cohort were examined for nine SNPs. Results: Maternal homozygosity of the rarer allele in the relaxin 2 gene (RLN2, rs10115467 and rs4742076) had an increased risk of moderate PTB (odds ratio 4.1 [95% CI 1.4-12] and 8.8 195% CI 1.03-75] respectively). Only rs10115467 remained significant after correction for multiple testing. Conclusion: Women homozygous for prevalent SNPs in the RLN2 gene may have a genetic susceptibility for PTB.