Mouse Lymphocytic choriomeningitis virus IgG ELISA Kit

Name: Mouse Lymphocytic choriomeningitis virus IgG ELISA Kit
SKU: DEIAJX009
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

The Mouse Anti-Lymphocytic Choriomeningitis Virus (LCMV) IgG ELISA Kit is an immunoassay suitable for detecting and quantifying IgG antibody activity specific for LCMV nuclear protein (NP) antigen in serum or plasma. Other biological fluids, including tissue culture medium, may be validated for use. The kit uses recombinant antigens and has no virus or viral proteins. For research use only.

Contents of Kit

1. Wash Solution Concentrate (100×), 10mL. Dilute the entire volume 10ml + 990ml with distilled or deionized water into a clean stock bottle. Label as Working Wash Solution and store at ambient temperature until kit is used entirely.
2. Sample Diluent Concentrate (20×), 10mL. Dilute the entire volume, 10ml + 190ml with distilled or deionized water into a clean stock bottle. Label as Working Sample Diluent and store at 2-8°C until the kit lot expires or is used up.
3. Anti-Mouse IgG-HRP Conjugate Concentrate (100×), 0.15mL. Peroxidase conjugated anti-Mouse IgG in buffer with detergents and antimicrobial as stabilizers. Dilute fresh as needed; 10ul of concentrate to 1ml of Working Sample Diluent is sufficient for 1 8-well strip. Use within the working day and discard. Return 100× to 2-8°C storage.
4. LCMV Ag Microwell Strip Plate, 8-well strips (12). Coated with LCMV VP1 antigen, and post-coated with stabilizers.
5. Mouse Anti-LCMV Sensitivity Control, 0.65 ml. Low level Mouse Anti-LCMV, in buffer with detergents and antimicrobial.
6. Mouse Anti-LCMV Positive Control, 0.65 ml. High level Mouse Anti-LCMV, in buffer with detergents and antimicrobial.
7. Low NSB Sample Diluent, 30 ml. Buffer with protein, detergents and anti-microbial. Use as is for sample dilution.
8. TMB Substrate, 12 ml. Chromogenic substrate for HRP containing TMB and peroxide.
9. Stop Solution, 12 ml. Dilute sulfuric acid.

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label. Stabilities of the working solutions are indicated under Reagent Preparation.

Precision

Samples and Controls were assayed in duplicate in 5 - 6 separate runs, to provide a measure of between-assay reproducibility.
The data are represented using the value of the Sensitivity Control in each assay to calculate a Threshold Index for each control and samples.

Results
The coefficient of variation (%CV) shows the reproducibility of the assay for measuring one antibody activity (sample or control) relative to another antibody activity (Sensitivity Control). Variation increases in the threshold region; for this reason, consider running additional tests for borderline samples.
Note:
The sensitivity of the assay may be increased to perhaps convert a borderline sample to a positive by using a lower dilution of the sample, e.g., 1/50. The values of negatives may increase, so an alternative threshold should be established using known negatives to develop a Positive Index, or by using known Internal Controls as discriminator for a Threshold Control (instead of the kit Sensitivity Control)

Sensitivity

The LCMV antigen coating level, HRP conjugate concentration, and Low NSB Sample Diluent are optimized to differentiate anti-LCMV IgG from background (non-antibody) signal with mouse serum samples diluted 1:100.

General Description

LCMV, an enveloped RNA arenavirus, is rare among laboratory animals, but is readily transmissible to lab workers. Infected mice generally do not present clinical signs. LCMV infection may be diagnosed by ELISA, measuring rapidly rising antibody titers (8-12 days after infection) to LCMV antigen. Mice infected with LCMV are not suitable for animal research due to suppression of cellular immunity, increased sensitivity to viruses and endotoxins, and inhibition of tumor induction or transplantation. Besides infecting animals, LCMV may also contaminate cell lines, transplantable tumors and other biological products; these should be tested by mouse antibody production (MAP), using ELISA to detect anti-LCMV after immunization.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Wild Red Foxes (Vulpes vulpes) as Sentinels of Rodent-borne Hantavirus and Lymphocytic Choriomeningitis Virus in the Province of Soria, Northern Spain

JOURNAL OF WILDLIFE DISEASES

Authors: Lledo, Lourdes; Luis Serrano, Jose; Gimenez-Pardo, Consuelo; Gegundez, Isabel

Abstract

Three hundred and fourteen red foxes (Vulpes vulpes) in the province of Soria, Spain, were examined for hantavirus and lymphocytic choriomeningitis virus (LCMV) infection (and were likely to have been infected by feeding on infected rodents). Immunofluorescence and western blot assays confirmed 3.5% (11/314) to have antibodies to hantaviruses, and the immune fluorescence assay showed 2.2% (7/314) to have antibodies to LCMV. The serologic status of the animals showed no statistically significant association with sex or age. Although studies on the prevalence of hantaviruses and LCMV normally focus on rodents, our results showed that foxes can provide complementary information in determined areas.

A functional subset of CD8(+) T cells during chronic exhaustion is defined by SIRP alpha expression

NATURE COMMUNICATIONS

Authors: Myers, Lara M.; Tal, Michal Caspi; Dulgeroff, Laughing Bear Torrez; Carmody, Aaron B.; Messer, Ronald J.; Gulati, Gunsagar; Yiu, Ying Ying; Staron, Matthew M.; Angel, Cesar Lopez; Sinha, Rahul; Markovic, Maxim; Pham, Edward A.; Fram, Benjamin; Ahmed, Aijaz; Newman, Aaron M.; Glenn, Jeffrey S.; Davis, Mark M.; Kaech, Susan M.; Weissman, Irving L.; Hasenkrug, Kim J.

Abstract

Prolonged exposure of CD8(+) T cells to antigenic stimulation, as in chronic viral infections, leads to a state of diminished function termed exhaustion. We now demonstrate that even during exhaustion there is a subset of functional CD8(+) T cells defined by surface expression of SIRP alpha, a protein not previously reported on lymphocytes. On SIRP alpha(+) CD8(+) T cells, expression of co-inhibitory receptors is counterbalanced by expression of co-stimulatory receptors and it is only SIRP alpha(+) cells that actively proliferate, transcribe IFN gamma and show cytolytic activity. Furthermore, target cells that express the ligand for SIRP alpha, CD47, are more susceptible to CD8(+) T cell-killing in vivo. SIRP alpha(+) CD8(+) T cells are evident in mice infected with Friend retrovirus, LCMV Clone 13, and in patients with chronic HCV infections. Furthermore, therapeutic blockade of PD-L1 to reinvigorate CD8(+) T cells during chronic infection expands the cytotoxic subset of SIRP alpha(+) CD8(+) T cells.