G. Pig Lymphocytic choriomeningitis virus Antibody ELISA Kit

Name: G. Pig Lymphocytic choriomeningitis virus Antibody ELISA Kit
SKU: DEIAJX008
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum and other biological fluids

Species Reactivity

Pig

Intended Use

The G. Pig Anti-Lymphocytic Choriomeningitis Virus IgG ELISA Kit is an immunoassay suitable for detecting and quantifying IgG antibody activity specific for LCMV nuclear protein (NP) antigen in serum or plasma. Other biological fluids, including tissue culture medium, may be validated for use. The kit use recombinant antigens and has no virus or viral proteins to avoid virus infection. For research use only (RUO).

Storage

2-8°C until the expiration date printed on the box label.

Precision

Samples and Controls were assayed in duplicate in 5 - 6 separate runs, to provide a measure of between-assay reproducibility.
The data are represented using the value of the Sensitivity Control in each assay to calculate a Threshold Index for each control and samples.

The coefficient of variation (%CV) shows the reproducibility of the assay for measuring one antibody activity (sample or control) relative to another antibody activity (Sensitivity Control). Variation increases in the threshold region; for this reason, consider running additional tests for borderline samples.

Sensitivity

The LCMV antigen coating level, HRP conjugate concentration, and Low NSB Sample Diluent are optimized to differentiate antiLCMV IgG from background (non-antibody) signal with G. Pig serum samples diluted 1:100.

General Description

LCMV, an enveloped RNA arenavirus, is rare among laboratory animals, but is readily transmissible to lab workers. Infected mice generally do not present clinical signs. LCMV infection may be diagnosed by ELISA, measuring rapidly rising antibody titers (8-12 days after infection) to LCMV antigen. Mice infected with LCMV are not suitable for animal research due to suppression of cellular immunity, increased sensitivity to viruses and endotoxins, and inhibition of tumor induction or transplantation. Besides infecting animals, LCMV may also contaminate cell lines, transplantable tumors and other biological products; these should be tested by mouse antibody production (MAP), using ELISA to detect anti-LCMV after immunization.

RecombiVirus ELISAs are 2nd generation immunoassays using purified recombinant, antigenic proteins of animal viruses. The Creative Diagnostics G.Pig LCMV IgG ELISA is designed with high sensitivity for the detection of antibodies derived from vaccination or natural LCMV infection.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Performance Comparison of FA, PSO and CS application in SINR Optimisation for LCMV Beamforming Technique

WIRELESS PERSONAL COMMUNICATIONS

Authors: Doroody, Camellia; Kiong, Tiong Sieh

Abstract

A beamforming technique called Linearly Constraint Minimum Variance (LCMV) allows directing a radiation beam towards the desired direction to minimise interference of the signal through weight vectors that are computed by LCMV. Generally, to achieve a favourable beam shape, LCMV's weights are not exactly steered towards the user's direction. In addition, traditional methods are not equipped well to seamlessly improve the weights of LCMV. This paper employs Particle Swarm Optimisation (PSO), Firefly Algorithm (FA) and Cuckoo Search (CS) to optimise the weights of LCMV. The key anticipated goal in LCMV optimisation is the power reduction on the interferences' side to achieve a favourable beam shape and better SINR output. A common metaheuristic algorithm is Particle Swarm Optimisation (PSO), which deals with the social behaviour of creatures such as bird flocking. A population and attraction-based algorithm is employed in Firefly algorithm; the flashing characteristics of fireflies are the inspiration of the swarm intelligence metaheuristic algorithm. Also, a novel equation-based nature inspired algorithm is Cuckoo Search (CS), which is based on the brood parasitism of a few cuckoo species combined with the so-called Levy flights. Based on simulation results, FA showed enhanced ability to precisely determine power allocation's optimal direction when compared with CS and PSO. Thus, better SINR results could be achieved with FA. For SINR optimisation using the LCMV technique, the effectiveness of CS in comparison with FA and PSO algorithms was simulated employing MATLAB (R).

Preceding Viral Infections Do Not Imprint Long-Term Changes in Regulatory T Cell Function

SCIENTIFIC REPORTS

Authors: Rost, Felix; Lambert, Katharina; Rakebrandt, Nikolas; Joller, Nicole

Abstract

Regulatory T cells (T-regs) maintain peripheral self-tolerance and limit immune mediated pathology. Like effector T cells, T-regs can specialize in T(H)1-dominated immune responses and co-express T-bet together with Foxp3. This allows for expression of CXCR3 and efficient homing to sites of T(H)1 responses. However, whether such functional specialization is paralleled by memory generation among T-regs is unknown. In this study, we investigated the ability of polyclonal T-regs to form functional memory in response to viral infection. Using adoptive transfer models to compare infection-experienced T-regs generated upon acute Lymphocytic Choriomeningitis Virus (LCMV) WE and Vaccinia Virus (VV) infections with naive T-regs, we observed no differences in their phenotype or their in vivo maintenance. When comparing functional properties of infection-experienced and naive T-regs, we found no differences in in vitro suppressive capacity nor in their ability to limit the effector response upon homologous, systemic or local re-challenge in vivo. Our results suggest that no functional T-reg memory is generated in the context of systemic LCMV or VV infection, but we cannot rule out the possibility that the generation of T-reg memory may be possible in other contexts.