Mouse FCRN/FCGRT ELISA Kit

Name: Mouse FCRN/FCGRT ELISA Kit
SKU: DEIABL623
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates

Species Reactivity

Mouse

Detection Method

sELISA

Intended Use

For quantitative detection of Mouse Fcgrt in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates.

Contents of Kit

Storage

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Precision

CD spends great efforts in documenting lot-to-lot variability and ensuring our assay kits produce robust data that are reproducible.
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Detection Range

93.7 pg/ml - 6,000 pg/ml

Sensitivity

<10 pg/ml

General Description

The neonatal Fc receptor (FcRn), also known as the Brambell receptor, is a protein that in humans is encoded by the FCGRT gene. The neonatal Fc receptor is an Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. It was first discovered in rodents as a unique receptor capable of transporting IgG from mother's milk across the epithelium of newborn rodent's gut into the newborn's bloodstream. Further studies revealed a similar receptor in humans, leading to the naming as a neonatal Fc receptor. In humans, however, it is found in the placenta to help facilitate transport of mother's IgG to the growing fetus and it has also been shown to play a role in monitoring IgG turnover. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine,TNF-α, and down-regulated by IFN-γ.

Standard Curve

The highest O.D. value might be higher or lower than in the example. The experiment result is statistically significant if the highest O.D. value is no less than 1.0.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Functional polymorphisms in rhesus macaque FCGRT and beta 2-m

IMMUNOGENETICS

Authors: Shubin, Zhanna; Tagaya, Yutaka; Poonia, Bhawna

Abstract

Rhesus macaque is an important animal model for studies testing interventions like antibody therapeutics; as such knowledge of inter-individual variations in function of genes affecting antibody recycling is important for optimal experimental design. Neonatal Fc receptor (FcRn), a heterodimer composed of FCGRT and beta 2-m chains, plays critical role in extending catabolic half-life of IgG. We studied genomic polymorphisms in rhesus macaque FcRn and asked if they are functional by assessing correlations with serum IgG or beta 2-m levels. We tested 75 animals and report the presence of a VNTR polymorphism in promoter of FcRn as well as a single nucleotide polymorphism in the signal peptide of beta 2-m. A VNTR minor allele was associated with lower levels of serum IgG. This polymorphism may account for inter-animal variation in antibody levels and has relevance for effective design of rhesus macaque studies investigating vaccine-induced antibody responses and passive immunizations.

ASSOCIATION OF FcRn HEAVY CHAIN ENCODING GENE (FCGRT) POLYMORPHISMS WITH IgG CONTENT IN BOVINE COLOSTRUM

ANIMAL BIOTECHNOLOGY

Authors: Zhang, Ran; Zhao, Zhihui; Zhao, Yaofeng; Kacskovics, Imre; van der Eijk, Marjolein; de Groot, Nanda; Li, Ning; Hammarstrom, Lennart

Abstract

In this study, we evaluated haplotypes of the bovine FCGRT (encoding the FcRn heavy chain) and their relationship to the IgG concentration in bovine colostrum. Four single nucleotide polymorphism (SNP), classified into five haplotypes, were identified in a total of 49 Holstein-Frisians cows. Haplotype 5 was found to be significantly associated with a high IgG level ([OR]-9.90, 95%CI-1.11-88.34, p-0.016) and haplotype 2 exhibited a similar trend ([OR]=2.89, 95%CI=1.17-7.11, p=0.019).