Mouse Complement 1q ELISA Kit

Sample

Serum, plasma, other biological fluids

Species Reactivity

Mouse

Detection Method

sELISA

Intended Use

This ELISA kit used for in vitro quantitative determination of C1q in mouse serum, plasma and other biological fluids. For research use only, and it's highly recommended to read thoroughly of this manual before using the product.

Contents of Kit

Storage

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Standard Lyophilized, Concentrated Biotin Conjugate Antibody, Streptavidin-HRP Concentrated and the Antibody Coated Plate should be stored at -20°C upon receipt while the others should be at 2-8 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
Note:
It is highly recommended to use the remaining reagents within 1 month provided this is prior to the expiration date of the kit. For the expiration date of the kit, please refer to the label on the kit box. All components are stable up to the expiration date.

Precision

Intra-plate Precision
3 samples with low, middle and high level C1q were tested 20 times on one plate, respectively.
Intra-Assay: CV<10%
Inter-plate Precision
3 samples with low, middle and high level C1q were tested on 3 different plates, 8 replicates in each plate.
Inter-Assay: CV<12%

Sensitivity

The minimum detectable dose (MDD) of C1q is typically less than 0.244 ng/mL. The MDD was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Standard Curve

The standard curves are provided for demonstration only. A standard curve should be generated for each set of C1q assayed.