Kynurenic Acid ELISA kit

Sample

Serum

Detection Method

cELISA

Intended Use

The Kynurenic Acid ELISA kit is optimized for the quantitation of Kynurenic Acid (KYNA) within serum samples. This easy-to-use competitive assay is well-suited for both small and large series of samples. Working with a 50 μL sample volume, this assay enables pre-clinical and clinical sample testings.

Contents of Kit

1. Adhesive Foil, Ready to use, 1 x 4 foils
2. Extraction Plate, Ready to use, 1 x 96 well plate
3. ELISA Wash Buffer Concentrate 50x, Buffer with a non-ionic detergent and physiological pH, 1 x 20 mL/vial, light purple cap
4. Enzyme Conjugate, Ready to use, Goat anti-rabbit immunoglobulins conjugated with peroxidase, 1 x 12 mL/vial, red cap
5. Substrate, Ready to use, Chromogenic substrate containing tetramethylbenzidine, substrate buffer and hydrogen peroxide, 1 x 12 mL/black vial, black cap
6. Stop Solution, Ready to use, 0.25 M sulfuric acid, 1 x 12 mL/vial, light grey cap, Hazards identification: H290 May be corrosive to metals.
7. Kynurenic acid Microtiter Strips, Ready to use, 1 x 96 well (12x8), antigen precoated microwell plate in a resealable pouch with desiccant
8. Kynurenic acid Antiserum Concentrate 2x, Rabbit anti-kynurenic acid antibody, blue coloured, 1 x 7 mL/vial, blue cap
9. Kynurenic acid Antiserum Diluent, Ready to use, 1 x 7 mL/vial, orange cap
10. Extraction Reagent, Ready to use, Buffer with acidic pH, 1 x 4 mL/vial, dark green cap, Hazards identification : H290 May be corrosive to metals.
11. Extraction Wash Buffer, Ready to use, Buffer with acidic pH, 1 x 35 mL/vial, white cap
12. Acylation Reagent, Lyophilized, 2 vials, dark green cap
13. Acylation Buffer, Ready to use, 2-(N-Morpholino)ethanesulfonic acid (MES) buffer, 2 x 30 mL/vial, brown cap
14. Cleaning Buffer, Ready to use, Buffer with acidic pH, 1 x 35 mL/vial, light yellow cap
15. Assay Buffer, Ready to use, Buffer with acidic pH, 1 x 35 mL/vial, dark blue cap, Hazards identification: H290 May be corrosive to metals.
16. Standards and Controls, Ready to use, 4 mL/vial
- Standard A, white, 0 ng/mL(0 nmol/L)
- Standard B, light yellow, 1.89 ng/mL(10 nmol/L)
- Standard C, orange, 4.73 ng/mL(25 nmol/L)
- Standard D, dark blue, 11.82 ng/mL(62.5 nmol/L)
- Standard E, light grey, 29.51 ng/mL(156 nmol/L)
- Standard F, black, 73.97 ng/mL(391 nmol/L)
- Control 1, light green, Refer to QC-Report for expected value and acceptable range.
- Control 2, dark red, Refer to QC-Report for expected value and acceptable range.
Conversion: Kynurenic acid (ng/mL) x 5.286 = Kynurenic acid (nmol/L)
Contents: Buffer with non-mercury stabilizer, spiked with defined quantity of Kynurenic acid

Storage

Store the unopened reagents at 2 - 8 C until expiration date. Do not use components beyond the expiry date indicated on the kit labels. Once opened the reagents are stable for 1 month when stored at 2 – 8 °C. Once the resealable pouch has been opened, care should be taken to close it tightly with desiccant
again.

Performance Characteristics

Detection Range

1.89 - 74 ng/mL

The calibration curve is obtained by plotting the absorbance readings (calculate the mean absorbance) of the standards (linear, y-axis) against the corresponding standard concentrations (logarithmic, x-axis).
Use non-linear regression for curve fitting (e.g. spline, 4- parameter, akima).
This assay is a competitive assay. This means: the OD-values are decreasing with increasing concentrations of the analyte. OD-values found below the standard curve correspond to high concentrations of the analyte in the sample.
The concentrations of the samples and controls can be read directly from the standard curve.

Sensitivity

0.53 ng/mL

General Description

Kynurenic acid (KYNA) is a downstream metabolite produced along the Kynurenine Pathway well-known to possess several neuromodulatory functions, as well as immuno-modulatory properties. Measurement of KYNA in biological samples has been presented as a possible biomarker in several diseases including neurological or psychiatric disorders.