Ibuprofen ELISA Kit

Sample

Urine, Blood, Oral Fluid

Species Reactivity

Human; Research Animal

Detection Method

cELISA

Intended Use

For the determination of trace quantities of Ibuprofen and/or other metabolites in samples.

Contents of Kit

1. EIA Buffer: 40 mL (ready-to-use). Phosphate buffered saline solution with bovine serum and a preservative. Provided for dilution of drug-enzyme conjugate concentrate and samples.
2. Wash Buffer Concentrate (10×): 20 mL. Phosphate buffered saline solution with a surfactant. Dilute 10 fold with deionized or ultrapure water before use. Diluted wash buffer is used to wash all unbound conjugate and samples from the plate after the conjugate incubation.
3. Substrate: 20 mL (ready-to-use). Stabilized 3, 3', 5, 5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H₂O₂) in a single bottle. It is used to develop the color in the wells after washing. Light sensitive.
4. Drug-Enzyme Conjugate: 200 μL. Drug-horseradish peroxidase concentrate. Dilute 180X before use.
5. Antibody Coated Plate: A 96 well Costar plate, in strips of 8 break-away wells, coated with anti-drug antiserum. The plate is ready for use as is. Do not wash.
6. Red Stop Solution: 20 mL (ready-to-use). Non-acidic reagent used to stop the enzyme reaction.
7. Qualitative QC Positive Control: 750 μL provided (synthetic human urine). Do not dilute.
8. Qualitative QC Negative Control: 750 μL provided (synthetic human urine). Do not dilute.

Storage

This kit can be used until the expiration date on the label when stored refrigerated at 2-8°C. Store controls frozen if not used within 10 days. Note: Some kits require controls to be stored frozen immediately upon receipt. Reference kit label for details.

Sensitivity

The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.

General Description

Creative Diagnostics's Ibuprofen ELISA (Enzyme-Linked ImmunoSorbent Assay) test kit is a qualitative one-step kit designed for use as a screening device for the detection of drugs and/or their metabolites. The kit was designed for screening purposes and is intended for research use only. It is recommended that all suspect samples be confirmed by a quantitative method such as gas chromatography/mass spectrometry (GC/MS).

Standard Curve