Product Overview
A DNA sequence encoding the Influenza A virus (A/equine/Kentucky/1a/ 1975(H7N7)) hemagglutinin (ACL12085.1) (Met1-Ile534) was expressed with a C-terminal polyhistidine tag.
Molecular Weight
The recombinant hemagglutinin of Influenza A virus (A/equine/Kentucky/ 1a/1975(H7N7)) comprises 545 amino acids and has a predicted molecular mass of 61.6 kDa. The apparent molecular mass of the protein is approximately 63.3 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 85 % as determined by SDS-PAGE
Format
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0.1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H7N7 HA;H7N7 HA; IAV H7N7 HA
Citations
Publication ()
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COA
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