A DNA sequence encoding the extracellular domain of Influenza A virus (A/chicken/Netherlands/1/03 (H7N7)) (AAR02639.1) hemagglutinin (Met 8-Phe 544) (Native, HA1+HA2) was expressed, fused with a polyhistidine tag at the C-terminus.
Nature
Recombinant
Tag/Conjugate
His
Molecular Weight
The secreted recombinant hemagglutinin of Influenza A virus (A/chicken/Netherlands/1/03 (H7N7)) comprises 530 amino acids and has a calculated molecular mass of 59.4 kDa as estimated in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 80 % as determined by SDS-PAGE
Format
Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -70°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H7N7 HA;H7N7 HA; IAV H7N7 HA
Citations
Publication ()
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