Product Overview
A DNA sequence encoding the Influenza A virus (A/Hong Kong/4801/2014 (H3N2)) nucleoprotein (translated amino acids of EPI653194) (Met1-Asn498) was expressed with a polyhistidine tag at the C-terminus.
Molecular Weight
The recombinant nucleoprotein of Influenza A virus (A/Hong Kong/4801/2014 (H3N2)) consists of 509 amino acids and predicts a molecular mass of 57.4 kDa.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Alternative Names
Influenzavirus A; Influenza A virus; Influenza A virus H3N2; H3N2; IAV H3N2; IAV H3N2 Nucleoprotein; IAV H3N2 NP; H3N2 NP; H3N2 Nucleoprotein
Purity
> 95 % as determined by SDS-PAGE.
Buffer
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Introduction
Influenza A viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H3N2; H3N2; IAV H3N2; IAV H3N2 Nucleoprotein; IAV H3N2 NP; H3N2 NP; H3N2 Nucleoprotein
Citations
Publication ()
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COA
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