Product Overview
A DNA sequence encoding the Influenza A virus (A/Michigan/45/2015 (H1N1pdm09)) neuraminidase (translated amino acids of EPI859652) (His36-Lys469) was expressed with a vasodilator-stimulated phosphoprotein tetramerization domain at the N-terminusa and a polyhistidine tag at the C-terminus.
Molecular Weight
The recombinant Influenza A virus (A/Michigan/45/2015 (H1N1pdm09)) NA consists of 480 amino acids and predicts a molecular mass of 53.6 kDa. The apparent molecular mass of the protein is approximately 62.6 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Alternative Names
Influenzavirus A; Influenza A virus; Influenza A virus H1N1; H1N1; IAV H1N1; IAV H1N1 Neuraminidase ; IAV H1N1 NA; H1N1 NA; H1N1 Neuraminidase
Purity
> 90 % as determined by SDS-PAGE
Buffer
Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 10 % glycerol, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Introduction
Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus.
Keywords
Influenzavirus A; Influenza A virus; Influenza A virus H1N1; H1N1; IAV H1N1; IAV H1N1 Neuraminidase ; IAV H1N1 NA; H1N1 NA; H1N1 Neuraminidase
Citations
Publication ()
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COA
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