Human Zonulin ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

cELISA

Intended Use

This ELISA is intended for the quantitative determination of zonulin family peptides (ZFP) in serum.

Contents of Kit

1. PLATE: Microtiter plate, pre-coated, 12 × 8 wells
2. WASHBUF: Wash buffer concentrate, 10×, 2 × 100 ml
3. DIL: Dilution buffer, ready-to-use, 1 × 100 ml
4. TRACER: Tracer concentrate, biotinylated ZFP, 1 × 300 μl
5. CONJ: Conjugate concentrate, peroxidase-labelled streptavidin, 1 × 200 μl
6. STD: Standards, lyophilized (see specification for concentrations), 4 × 5 vials
7. CTRL1: Control 1, lyophilized (see specification for range), 4 × 1 vial
8. CTRL2: Control 2, lyophilized (see specification for range), 4 × 1 vial
9. SUB: Substrate, (tetramethylbenzidine), ready-to-use, 15 ml
10. STOP: Stop solution, ready-to-use, 15 ml

Storage

2-8°C

Precision

Intra-Assay: CV < 8% (n = 40)
Inter-Assay: CV < 10% (n = 25)

Detection Range

0.25-16 ng/ml

Sensitivity

The following values have been estimated based on the concentrations of the standard without considering possibly used sample dilution factors:
Limit of blank, LoB 0.140 ng/ml
Limit of detection, LoD 0.183 ng/ml
Limit of quantitation, LoQ 0.183 ng/ml
The evaluation was performed according to the CLSI guideline EP17-A2. The specified accuracy goal for the LoQ was 20% CV.