Human Zonulin ELISA Kit (DEIA2225)
Size
96T
Sample
serum, plasma, saliva, urine, serum-free cell culture supernates
Species Reactivity
Human
Intended Use
For the quantitative determination of human Haptoglobin concentrations in serum-free cell culture supernates, serum, plasma, saliva, and urine.
Contents of Kit
1. Human Haptoglobin Microplate: 96 well polystyrene microplate (12 strips of 8 wells)
2. Human Haptoglobin Conjugate: 21 mL
3. Human Haptoglobin Standard: 400 ng
4. Assay Diluent CD2-210: 2 vials (11 mL/vial)
5. Calibrator Diluent CD6-78: 21 mL
6. Wash Buffer Concentrate: 21 mL
7. Color Reagent A: 12 mL
8. Color Reagent B: 12 mL
9. Stop Solution: 6 mL
10. Plate Sealers: 4 adhesive strips
2. Human Haptoglobin Conjugate: 21 mL
3. Human Haptoglobin Standard: 400 ng
4. Assay Diluent CD2-210: 2 vials (11 mL/vial)
5. Calibrator Diluent CD6-78: 21 mL
6. Wash Buffer Concentrate: 21 mL
7. Color Reagent A: 12 mL
8. Color Reagent B: 12 mL
9. Stop Solution: 6 mL
10. Plate Sealers: 4 adhesive strips
Storage
Store the unopened kit at 2-8°C. Do not use past kit expiration date.
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Sensitivity
Twenty-five assays were evaluated and the minimum detectable dose (MDD) of human Haptoglobin ranged from 0.031-0.529 ng/mL. The mean MDD was 0.192 ng/mL.
The MDD was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
The MDD was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Standard Curve
Citations
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