Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Human RNLS (Renalase) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.78-50 ng/mL
Citations
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Boomsma, F; Tipton, KF; et al. Renalase, a catecholamine-metabolising enzyme?. JOURNAL OF NEURAL TRANSMISSION 114:775-776(2007).
Howson, JMM; Cooper, JD; et al. Evidence of Gene-Gene Interaction and Age-at-Diagnosis Effects in Type 1 Diabetes. DIABETES 61:3012-3017(2012).