Human PIIANP (Type IIA Collagen N-Propeptide) ELISA Kit

Sample

Serum, plasma, tissue homogenates and other biological fluids

Species Reactivity

Human

Detection Method

cELISA

Intended Use

DEIA7442R is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PIIANP (Type IIA Collagen N-Propeptide) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PIIANP. The ELISA analytical biochemical technique of the DEIA7442R kit is based on PIIANP antibody-PIIANP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PIIANP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PIIANP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Contents of Kit

1.Pre-coated Microplate, 12 strips x 8 wells, 4°C
2.Standard (lyophilized), 2, 4°C
3.Standard Diluent Buffer, 20 mL, 4°C
4.Biotinylated-Conjugate (100x), 60 μL, 4°C
5.Biotinylated Conjugate Diluent, 10 mL, 4°C
6.Streptavidin-HRP(100×), 120 μL, 4°C
7.HRP Diluent, 12 mL, 4°C
8.Wash Buffer (25×), 20 mL, 4°C
9.TMB Substrate Solution, 9 mL, 4°C(lucifuge)
10.Stop reagent, 6 mL, 4°C
11.Plate Covers, 2, 4°C
12.Instruction manual, 1, 4°C

Storage

1. Store kit at 4°C immediately upon receipt.
2. Do not use the kit after the expiration date.
3. Please check whether all components are complete after opening the package.
All kit components have been formulated and quality control tested to function successfully as a kit. Do not mix or substitute reagents or materials from other kit, performance cannot be guaranteed if utilized separately or substituted.

Precision

Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Detection Range

15.6-1000 ng/mL

Sensitivity

5.9 ng/mL

Standard Curve

Note: this graph is for reference only.