Sample
serum, plasma, cell culture supernates, cell lysates, tissue homogenates or other biological fluids
Intended Use
This kit is for the qualitative detection of Human Anti-Neisseria Gonorrhoeae Antibody,NGH Ab in serum, plasma, cell culture supernates, cell lysates, tissue homogenates or other biological fluids.This product is for research use only, not for use in diagnosis procedures. It's highly recommend to read this instruction entirely before the use.
Contents of Kit
| No. | Components | Size | Storage Conditions |
| 1 | Pre-coated Plate | 12 × 8 well strips × 1 | 2-8°C/ -20°C |
| 2 | Positive Control | 0.5ml × 1 vial | 2-8°C/ -20°C |
| 3 | Negative Control | 0.5ml × 1 vial | 2-8°C/ -20°C |
| 4 | HRP Conjugated | 6ml × 1 vial | 2-8°C/ -20°C |
| 5 | Sample Diluent | 6ml × 1 vial | 2-8°C/ -20°C |
| 6 | Substrate Solution A | 6ml ×1 vial | 2-8°C/ -20°C |
| 7 | Substrate Solution B | 6ml ×1 vial | 2-8°C/ -20°C |
| 8 | Stop Solution | 6ml ×1 vial | 2-8°C/ -20°C |
| 9 | Wash Buffer (25×) | 20ml × 1 vial | 2-8°C/ -20°C |
| 10 | Plate Sealer | 2 pcs | |
| 11 | Zipper Bag | 1 | |
| 12 | User Instruction | 1 | |
Storage
Store the reagents at 2-8°C. For long term storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
CV(%) = SD/mean x 100
Intra-Assay: CV< 10%
Inter-Assay: CV< 12%
General Description
Gonorrhea (colloquially known as the clap) is a common human sexually transmitted infection caused by the bacterium Neisseria gonorrhoeae. The usual symptoms in men are burning with urination and penile discharge. Women, on the other hand, are asymptomatic half the time or have vaginal discharge and pelvic pain. In both men and women if gonorrhea is left untreated, it may spread locally causing epididymitis or pelvic inflammatory disease or throughout the body, affecting joints and heart valves. Treatment is commonly with ceftriaxone as antibiotic resistance has developed to many previously used medications. This is typically given in combination with either azithromycin or doxycycline. There have been some strains of gonorrhea showing resistance to ceftriaxone.
General Notes
Gonorrhea (colloquially known as the clap) is a common human sexually transmitted infection caused by the bacterium Neisseria gonorrhoeae. The usual symptoms in men are burning with urination and penile discharge. Women, on the other hand, are asymptomatic half the time or have vaginal discharge and pelvic pain. In both men and women if gonorrhea is left untreated, it may spread locally causing epididymitis or pelvic inflammatory disease or throughout the body, affecting joints and heart valves. Treatment is commonly with ceftriaxone as antibiotic resistance has developed to many previously used medications. This is typically given in combination with either azithromycin or doxycycline. There have been some strains of gonorrhea showing resistance to ceftriaxone.
Citations
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