Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Human MYH11 (Myosin-11) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.78-50 ng/mL
Citations
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McKenna, M; Arnold, C; et al. Myeloid sarcoma of the small bowel associated with a CBF beta/MYH11 fusion and inv(16)(p13q22): a case report. JOURNAL OF CLINICAL PATHOLOGY 62:757-759(2009).
Dekking, E; van der Velden, VHJ; et al. Detection of fusion genes at the protein level in leukemia patients via the flow cytometric immunobead assay. BEST PRACTICE & RESEARCH CLINICAL HAEMATOLOGY 23:333-345(2010).