Human MPEG1 (Macrophage-expressed gene 1 protein) ELISA Kit

An algorithm for detecting scene changes in compressed video streams is proposed. The proposed algorithm directly exploits the motion compensation information and the prediction error signal in the MPEG1-coded video stream. By performing minimal decoding of the compressed video stream, the proposed algorithm results in significant savings in terms of excecution time and memory requirement. Experimental results show that the algorithm is effective in detecting abrupt scene changes (cuts) as well as gradual scene changes (dissolves) in an MPEG1-coded video stream. The algorithm is capable of processing video frames in real time and could be used for the rapid generation of key frames for real-time browsing of video streams and for indexing to support content-based access to on-line video libraries.

Perforin-2, the product of the MPEG1 gene, limits the spread and dissemination of bacterial pathogens in vivo. It is highly expressed in murine and human phagocytes, and macrophages lacking Perforin-2 are compromised in their ability to kill phagocytosed bacteria. In this study, we used Salmonella enterica serovar Typhimurium as a model intracellular pathogen to elucidate the mechanism of Perforin-2's bactericidal activity. In vitro Perforin-2 was found to facilitate the degradation of Ags contained within the envelope of phagocytosed bacteria. In contrast, degradation of a representative surface Ag was found to be independent of Perforin-2. Consistent with our in vitro results, a protease-sensitive, periplasmic superoxide dismutase (SodCII) contributed to the virulence of S. Typhimurium in Perforin-2 knockout but not wild-type mice. In aggregate, our studies indicate that Perforin-2 breaches the envelope of phagocytosed bacteria, facilitating the delivery of proteases and other antimicrobial effectors to sites within the bacterial cell.