Low bit-rate video streaming for face-to-face teleconference
2004 IEEE INTERNATIONAL CONFERENCE ON MULTIMEDIA AND EXP (ICME), VOLS 1-3
Authors: Wen, Z; Liu, ZC; Cohen, M; Li, J; Zheng, K; Huang, T
Abstract
Face-to-face video teleconferencing is very important for real time communication. Current teleconferencing application uses standard video codec, such as MPEG1/2/4, for the compression of face video. It either requires high bandwidth for high quality video transmission, or the transmitted face video be blurred at low bit-rate. In this paper, we present a system for real-time coding of face video at low bit-rate. There are two main contributions. First, we improve the technique of long term memory prediction by selecting frames into the database in an optimal way. A new frame is selected into the database only when it is significantly different from those frames which are already in the database. In this way, the database can cover a wider range of images. Second, we incorporate the prior knowledge about faces into the long term memory prediction framework. The prior knowledge includes: (1) facial motions are repetitive such that most of them can be reconstructed from multiple reference frames; and (2) different components of the face and the background could tolerate different level of error because of different perceptual importance. Experiments show that at similar PSNR the proposed system works much faster and achieves better visual quality than standard H.264/JVT codec.
The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish
PLOS ONE
Authors: Walton, Eric M.; Cronan, Mark R.; Beerman, Rebecca W.; Tobin, David M.
Abstract
Transgenic labeling of innate immune cell lineages within the larval zebrafish allows for real-time, in vivo analyses of microbial pathogenesis within a vertebrate host. To date, labeling of zebrafish macrophages has been relatively limited, with the most specific expression coming from the mpeg1 promoter. However, mpeg1 transcription at both endogenous and transgenic loci becomes attenuated in the presence of intracellular pathogens, including Salmonella typhimurium and Mycobacterium marinum. Here, we describe mfap4 as a macrophage-specific promoter capable of producing transgenic lines in which transgene expression within larval macrophages remains stable throughout several days of infection. Additionally, we have developed a novel macrophage-specific Cre transgenic line under the control of mfap4, enabling macrophage-specific expression using existing floxed transgenic lines. These tools enrich the repertoire of transgenic lines and promoters available for studying zebrafish macrophage dynamics during infection and inflammation and add flexibility to the design of future macrophage-specific transgenic lines.