Application: We recommend the following antibodies for sandwich immunoassay (Capture - Detection): DMAB-CPT805 - DMAB-CPT806
Summary
Specifications
Antibody Isotype
IgG
Clone
351171E9
Species Reactivity
Human
Immunogen
MMP13 fusion protein
Conjugate
Unconjugated
Applications
Application Notes
We recommend the following antibodies for sandwich immunoassay (Capture - Detection): DMAB-CPT805 - DMAB-CPT806
General Notes
This product (Catalog No. DMAB-CPT805) is a rabbit-derived monoclonal antibody raised against human MMP13. It has been affinity purified by Protein A. MMP-13(Matrix metalloproteinase-13), cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial MMP-1. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme, along with a smaller band at approximately 30 kDa(PMID:10771523). It also can be detected 3 bands that the glycosylated proMMP-13 (70 kDa), unglycosylated form (55 kDa), and active MMP-13 enzyme (40kDa) by western blot. DMAB-CPT805 is suitable for use in CBA(Cap), ELISA(Cap) applications. DMAB-CPT805 is supplied as a liquid formulation in PBS only. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
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