Human IFN-γ ELISpot Kit (with EBV peptide pool)

Sample

Cells

Species Reactivity

Human

Detection Method

ELISpot

Intended Use

The kit is a highly sensitive method used to detect and quantify individual cells that secrete IFN-γ after stimulation with the EBV Peptide Pool. This assay is utilized in immunology to monitor cellular immune responses at the single-cell level and reliably detects and measures human IFN-γ secretion by EBV stimulated effector cells.

Contents of Kit

1. Peptide Pools​: Human EBV (44 peptides from Human herpesvirus 4 (Epstein Barr virus))
2. Plates​: ELISpot plate precoated with IFN-γ capture antibody
3. Detection mAb​​: Biotinylated recombinant IFN-γ antibody
4. Enzyme conjugate​: Streptavidin-ALP (Alkaline Phosphatase)
5. Substrate: BCIP/NBT Ready-to-use solution
6. Blocking agent: Bovine Serum Albumin (BSA)

Storage

Store plates and reagents between 2 and 8°C. Peptide pool vial must be stored at -20 °C or below.

General Description

Epstein-Barr Virus (EBV), also known as human herpesvirus 4 (HHV-4), is a ubiquitous herpesvirus that infects around 90% of the human population. While primary EBV infection is often asymptomatic or presents as infectious mononucleosis, the reactivated virus is also associated with several malignancies, including Burkitt's lymphoma, Hodgkin's lymphoma, and nasopharyngeal carcinoma. Recent studies also suggest that EBV infection may also cause multiple sclerosis. Accurate diagnosis of EBV is important for identifying and managing both acute infections and EBV-associated malignancies, particularly for immunocompromised individuals who are likely to develop symptoms if EBV reactivates. Early detection of EBV-related diseases can guide therapeutic decisions and improve clinical outcomes for cohorts who are at higher risk for severe disease. Research indicates that individuals with strong EBV-specific T cell responses are less likely to develop severe complications, emphasizing the importance of immune monitoring in EBV infections.