Specifications
Species Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide surrounding to the epitope -NVSYI- with a phosphorylation site at Tyr216 of human GSK3-beta protein. This sequence is identical among human, mouse, rat, bovine, dog and chicken species.
Applications
Application Notes
WB: 0.1-1 ug/mL; ELISA: 0.01-0.1 ug/mL; IP: 2-5 ug/mL; IHC: 2-10 ug/mL
Images
Western blot analysis of Phospho-GSK-3beta (pY216) on the EGF stimulated A431 cell lysates (20 ug/lane) with Rabbit anti GSK-3beta (pY216) (Cat# CABT-CS436) antibody at 1:500 dilution. Observed a major immunoreactive band at molecular weight ~46kDa.
Immunohistochemical analysis of paraffin-embedded human carcinoma stained with Rabbit anti GSK-3beta (pY216) (Cat# CABT-CS436) antibody at 1:200 dilution.
Target
Alternative Names
GSK3B; glycogen synthase kinase 3 beta; Glycogen Synthase Kinase 3b
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.
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Citations
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Coffa, B; Meng, X; et al. ETHANOL REGULATION OF GSK3B IN PFC. ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH 36:11A-11A(2012).
Gerbal-Chaloin, S; Raulet, E; et al. GSK3B INHIBITORS REGULATE CYP EXPRESSION IN PRIMARY HUMAN HEPATOCYTES BY MANY WAYS: ACTIVATION OF CTNNB1, AHR AND PXR. DRUG METABOLISM REVIEWS 45:142-143(2014).
COA
Certificate of Analysis
