Specifications
Species Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK3 beta
Applications
Application Notes
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
Images
ICC analysis of Phospho-GSK3 beta (Ser9)(green) in Hela using DMAB-JX2362058 and DAPI(blue)
WB analysis of Phospho-GSK3 beta (Ser9) in K562, C6, 3T3, Hela lysates using DMAB-JX2362058.
IHC analysis of paraffin-embedded Human colon cancer using DMAB-JX2362058. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Western blot analysis of Phospho-GSK3 beta (Ser9) in K562, C6, 3T3, Hela lysates using Phospho-GSK3 beta (Ser9) antibody (DMAB-JX2362058).
Immunocytochemistry analysis of Phospho-GSK3 beta (Ser9)(green) in Hela using Phospho-GSK3 beta (Ser9) antibody (DMAB-JX2362058), and DAPI(blue)
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Phospho-GSK3 beta (Ser9) antibody (DMAB-JX2362058). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Target
Alternative Names
GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.
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Citations
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Das, ND; Jung, KH; et al. The Role of NF-kappa B and H3K27me3 Demethylase, Jmjd3, on the Anthrax Lethal Toxin Tolerance of RAW 264.7 Cells. PLOS ONE 5:-(2010).
Allen, JJ; Li, MQ; et al. A semisynthetic epitope for kinase substrates. NATURE METHODS 4:511-516(2007).