Human CXCL13 (C-X-C motif chemokine 13) ELISA Kit

Sample

Serum, plasma, cellculture supernatant, cell lysate or tissue lysate, other biological fluid samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of CXCL13 concentrations in Serum, plasma, cellculture supernatant, cell lysate or tissue lysate, other biological fluid samples.

Contents of Kit

The sealed kit can be stored at 2-8°C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foilbag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 monthat -20°C.
2. Lyophilized Standard, 2vial. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
3. Cap/Det Ab (Concentrated), 120μl. 2-8°C (Avoid Direct Light)
4. Antibody storage solution, 6ml. 2-8°C (Avoid Direct Light)
5. HRP-Streptavidin (Ready to use, orange), 10ml. 2-8°C (Avoid Direct Light)
6. TMB Substrate, 10ml. 2-8°C (Avoid Direct Light)
7. Sample Dilution Buffer (blue), 20ml. 2-8°C
8. Stop Solution, 5ml. 2-8°C
9. Wash Buffer(25×), 30ml. 2-8°C
10. Plate Sealer, 5 pieces
11. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Use amicropipette to measure accurately.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

15.625-1000pg/ml

Sensitivity

9.375pg/ml

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. (TMB was balanced to room temperature before color development, and incubated at 37°C for 15minutes in the dark after adding the enzyme label plate holes.) The following assay data are provided for reference, since experimental environment and operation aredifferent. The establishment of standard curve depends on your own assay.