Human CRABP2 ELISA Kit

Sample

Cell Lysates, Tissue Homogenates

Species Reactivity

Mouse

Detection Method

sELISA

Intended Use

The Kit is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human CRABP2 in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CRABP2 as low as 0.124 nanograms per millilter.

Storage

Upon receipt the kit should be stored at 4°C if intended for use within 24 hours. Otherwise the Assay Plate, Standard, Detection Reagent A, and Detection Reagent B should be stored at -20°C. Avoid repeated freeze-thaw cycles. Store all other kit components at 4°C. The Substrate should never be frozen. Once individual reagents are opened it is recommended that the kit be used within 1 month. Unused Strip Plate wells should be stored at -20°C in a sealed bag containing desiccant in order to minimize exposure to moisture. Do not use the kit beyond its expiration date.

Precision

Intra-Assay CV (<10%); Inter-Assay CV (<12%)

Detection Range

0.312–20 ng/ml

Sensitivity

Typically less than 0.124 ng/ml

Standard Curve

The following standard curve is an example only and should not be used to calculate results for tested samples. A new standard curve must be generated for each set of samples tested.