Human Cellular Retinoic Acid Binding Protein 2 (CRABP2) ELISA Kit

Sample

Tissue homogenates, cell lysates or other biological fluids

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CRABP2 in Human tissue homogenates, cell lysates or other biological fluids.

Storage

For unopened kits: All the reagents should be kept according to labels on the vials. The TMB Substrate, Wash Buffer (30× concentrate) and the Stop Solution should be stored at 4°C upon receipt while the others should be at -20°C.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Note: For the expiration date of the kit, please refer to the label on the kit box. All components are stable until this expiration date. It is highly recommended to use the remaining reagents within 1 month of opening.

Performance Characteristics

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CRABP2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CRABP2 were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/mean×100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Detection Range

0.312-20ng/mL. The standard curve concentrations used for the ELISA' s were 10, 10ng/mL, 10ng/mL, 10ng/mL, 10ng/mL, 10ng/mL, 10ng/mL.

Sensitivity

The minimum detectable dose of CRABP2 is typically less than 0.114ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Standard Curve

In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting the log of the data to establish a standard curve for each test is recommended. The typical standard curve below is provided for reference only.