Product Overview
A DNA sequence encoding the extracellular domain of human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N terminal
Gln 16
Molecular Weight
The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa.
In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant human CD64A is approximately 45-60 kDa due to glycosylation.
The molecular weight of this protein is around 38-52 kDa verified by SEC-MALS.
Bio-activity
1.Immobilized Anti-CD20(Ro) Antibody (Rituximab) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His Tag)(Cat:DAG-PY2602), the EC50 0.6-2.0 ng/mL.
2.Captured Human FcγRI / CD64 recombinant protein (Cat:DAG-PY2602) on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay.
3.Loaded Human FcγRI / CD64 recombinant protein (Cat:DAG-PY2602) on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay.
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Alternative Names
CD64; FCGR1A; FcγRI; FcγR1A; Fc Gamma Receptor Ia
Purity
≥ 95 % as determined by SDS-PAGE
≥ 90 % as determined by SEC-HPLC
≥ 90 % as determined by SEC-MALS
Buffer
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Introduction
FcγRI, also known as CD64 and FcR I, is a type I transmembrane protein, belonging to the immunoglobulin superfamily. FcγRI is an Fc receptor that binds to monomeric IgG with high affinity. Human FcγRI binds to human IgG1, IgG3 and IgG4 with higher affinity, but no affinity for IgG2. However, murine FcγRI only binds to mouse IgG2a with high affinity, but it binds to mouse IgG2b and IgG3 with low affinity and no affinity for mouse IgG1. Upon binding to IgG, Fc γRI associates with signal-transducing FcR common γ chain, and in turns conducts downstream signaling. Though the Fc γ chain is dispensable to FcγRI ligand-binding capacity, the surface expression of FcγRI and signaling are impaired in Fc γ chain-deficient cells. FcγRI is exclusively expressed on myeloid cells, including monocytes/macrophages, dendritic cells, and inducible on neutrophils and mast cells. The expression can be upregulated by IFN-γ, IL10 and G-CSF stimulation. Several studies demonstrated that FcγRI plays a role in antigen capture, IgG-induced cellular phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). In FcγRI-deficient mouse macrophages, the IgG2a-induced phagocytosis and antibody-mediated killing were impaired. In addition, acute and chronic inflammatory responses and immune complex-dependent antigen presentation to primed T cells were disturbed in Fcγ RI-deficient mice.
Keywords
CD64; FCGR1A; FcγRI; FcγR1A; Fc Gamma Receptor Ia
Citations
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COA
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