Recombinant Human CD300f/CD300LF Protein [His]

Name: Recombinant Human CD300f/CD300LF Protein [His]
SKU: DAGC297
Rating: 5 (1 reviews)

Recombinant Human CD300f/CD300LF Protein from HEK293 Cells [His]

COA

SDS

Datasheet

Summary

Product Overview

A DNA sequence encoding the human CD300LF (NP_620587.2) (Met1-Leu155) was expressed with a polyhistidine tag at the C-terminus.

Target

Human CD300LF

Nature

Recombinant

Predicted N terminal

Thr 20

Tag/Conjugate

His

Molecular Weight

The recombinant human CD300LF consists of 147 amino acids and predicts a molecular mass of 16.9 kDa.

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Alternative Names

CD300LF; CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300f; IREM-1; IgSF13; Human CD300LF

Purity

> 95 % as determined by SDS-PAGE.

Format

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Preservative

None

Storage

Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Introduction

Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:15549731). Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis (By similarity). Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands (PubMed:24035150). May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling (By similarity). Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:22043923). Inhibits osteoclast formation. Induces macrophage cell death upon engagement (By similarity).

Keywords

CD300LF; CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300f; IREM-1; IgSF13; Human CD300LF

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Tropism for tuft cells determines immune promotion of norovirus pathogenesis

SCIENCE

Authors: Wilen, Craig B.; Lee, Sanghyun; Hsieh, Leon L.; Orchard, Robert C.; Desai, Chandni; Hykes, Barry L., Jr.; McAllaster, Michael R.; Balce, Dale R.; Feehley, Taylor; Brestoff, Jonathan R.; Hickey, Christina A.; Yokoyama, Christine C.; Wang, Ya-Ting; MacDuff, Donna A.; Kreamalmayer, Darren; Howitt, Michael R.; Neil, Jessica A.; Cadwell, Ken; Allen, Paul M.; Handley, Scott A.; Campagne, Menno van Lookeren; Baldridge, Megan T.; Virgin, Herbert W.

Abstract

Complex interactions between host immunity and the microbiome regulate norovirus infection. However, the mechanism of host immune promotion of enteric virus infection remains obscure. The cellular tropism of noroviruses is also unknown. Recently, we identified CD300lf as a murine norovirus (MNoV) receptor. In this study, we have shown that tuft cells, a rare type of intestinal epithelial cell, express CD300lf and are the target cell for MNoV in the mouse intestine. We found that type 2 cytokines, which induce tuft cell proliferation, promote MNoV infection in vivo. These cytokines can replace the effect of commensal microbiota in promoting virus infection. Our work thus provides insight into how the immune system and microbes can coordinately promote enteric viral infection.

Norovirus Attachment and Entry

VIRUSES-BASEL

Authors: Graziano, Vincent R.; Wei, Jin; Wilen, Craig B.

Abstract

Human norovirus is a major human pathogen causing the majority of cases of viral gastroenteritis globally. Viral entry is the first step of the viral life cycle and is a significant determinant of cell tropism, host range, immune interactions, and pathogenesis. Bile salts and histo-blood group antigens are key mediators of norovirus entry; however, the molecular mechanisms by which these molecules promote infection and the identity of a potential human norovirus receptor remain unknown. Recently, there have been several important advances in norovirus entry biology including the identification of CD300lf as the receptor for murine norovirus and of the role of the minor capsid protein VP2 in viral genome release. Here, we will review the current understanding about norovirus attachment and entry and highlight important future directions.