Human CA14 (Carbonic anhydrase 14) ELISA Kit

Name: Human CA14 (Carbonic anhydrase 14) ELISA Kit
SKU: DEIA-FN200
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

serum, plasma, cell culture supernatants, tissue homogenate

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

For quantitative detection of Human CA14 (Carbonic anhydrase 14) in serum, plasma, tissue homogenates and other biological fluids.

Contents of Kit

1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Detection Range

0.156-10 ng/mL

Sensitivity

0.094 ng/mL

Standard Curve

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Charge dynamics of A(14)Cu(24)O(41) ladder compounds in two ends: Sr14 and Ca14

ADVANCES IN SUPERCONDUCTIVITY XII

Authors: Kojima, KM; Someya, M; Motoyama, N; Eisaki, H; Uchida, S; Furubayashi, Y; Terashima, T; Takano, M

Abstract

We report optical reflectivity measurement of the spin ladder compound A(14)Cu(24)O(41). Reflectivity of the purely A=Sr system (Sr14) exhibited a 'metallic' behavior in the E//c (//leg) polarization at room temperature, while below similar to 200K, there was a shift of the spectral weight from infrared to visible region. The increase of the chain Cu3+ Signal (omega similar to 3eV) suggests that some of the holes on the ladder move to the chain sites at low temperatures. We present a preliminary result of optical reflectivity of A=Ca compound (Ca14) as well, which is available only in a form of thin film evaporated onto Sr14 substrate.

Deletions of the Idh1, Eco1, Rom2, and Taf10 Genes Differently Control the Hyphal Growth, Drug Tolerance, and Virulence of Candida albicans

FOLIA BIOLOGICA

Authors: Hameed, A.; Hussain, S. A.; Ijaz, M. U.; Umer, M.

Abstract

The most recent genome-editing system called CRISPR-Cas9 (clustered regularly interspaced short palindromic repeat system with associated protein 9-nuclease) was employed to delete four non-essential genes (i.e., Caeco1, Caidh1, Carom2, and Cataf10) individually to establish their gene functionality annotations in pathogen Candida albicans. The biological roles of these genes were investigated with respect to the cell wall integrity and biogenesis, calcium/calcineurin pathways, susceptibility of mutants towards temperature, drugs and salts. All the mutants showed increased vulnerability compared to the wild-type background strain towards the cell wall-perturbing agents, (antifungal) drugs and salts. All the mutants also exhibited repressed and defective hyphal growth and smaller colony size than control CA14. The cell cycle of all the mutants decreased enormously except for those with Carom2 deletion. The budding index and budding size also increased for all mutants with altered bud shape. The disposition of the mutants towards cell wall-perturbing enzymes disclosed lower survival and more rapid cell wall lysis events than in wild types. The pathogenicity and virulence of the mutants was checked by adhesion assay, and strains lacking rom2 and eco1 were found to possess the least adhesion capacity, which is synonymous to their decreased pathogenicity and virulence.