Human Acyloxyacyl hydrolase (AOAH) ELISA kit

Sample

serum, plasma, tissue homogenates, cell lysates

Species Reactivity

Human

Detection Method

sELISA

Intended Use

This Human AOAH ELISA Kit was designed for the quantitative measurement of Human AOAH protein in serum, plasma, tissue homogenates, cell lysates. For research use only, not for use in diagnostic procedures.

Contents of Kit

1. Assay plate (12×8 coated Microwells), 1 (96 wells)
2. Standard (Freeze dried), 2
3. Biotin-antibody (100× concentrate), 1×120 μl
4. HRP-avidin (100× concentrate), 1×120 μl
5. Biotin-antibody Diluent, 1×15 ml
6. HRP-avidin Diluent, 1×15 ml
7. Sample Diluent, 1×50 ml
8. Wash Buffer (25×concentrate), 1×20 ml
9. TMB Substrate, 1×10 ml
10. Stop Solution, 1×10 ml
11. Adhesive Strip (For 96 wells), 4

Storage

Store at 2-8°C. Please refer to protocol.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

General Description

Acyloxyacyl hydrolase (AOAH) is a 2-subunit lipase which selectively hydrolyzes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial endotoxins. AOAH may modulate host inflammatory responses to gram-negative bacterial invasion. The 2 subunits are encoded by a single mRNA.
This Human AOAH ELISA Kit was designed for the quantitative measurement of Human AOAH protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.