Serum and other biological fluids
The Human Anti-Japanese Encephalitis Virus Prm Protein IgG ELISA Kit is an immunoassay suitable for detecting and quantifying IgG antibody activity specific for JEV envelope protein E in serum or plasma. Other biological fluids, including tissue culture medium, may be validated for use. This immunoassay is suitable for:
Determining immune status relative to non-immune controls;
Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing;
Qualifying and standardizing vaccine batches & protocols The kit contains NO virus or viral proteins. The assay is for research use only (RUO) and is not intended nor validated for diagnosing JEV. Reagents contain no virus.
2-8°C until the expiration date printed on the box label.
The JEV-coated plate, anti-Human IgG HRP concentration, and Low NSB Sample Diluent are optimized to differentiate anti-JEV IgG from immune and non-immune individuals when samples are tested at a dilution of 1:100 or higher.
Japanese encephalitis—previously known as Japanese B encephalitis to distinguish it from von Economo's A encephalitis—is a disease caused by the mosquito-borne Japanese encephalitis virus (JEV). Domestic pigs and wild birds are reservoirs of the virus; transmission to humans may cause severe symptoms. One of the most important vectors of this disease is the mosquito Culex tritaeniorhynchus. This disease is most prevalent in Southeast Asia and the Far East. Severe rigors mark the onset of this disease in humans. Signs which develop during the acute encephalitic stage include neck rigidity, cachexia, hemiparesis, convulsions and a raised body temperature between 38 and 41°C. Mental retardation developed from this disease usually leads to coma. Japanese Encephalitis is diagnosed by detection of antibodies in serum and CSF (cerebrospinal fluid) by ELISA.
JEV is closely related to the West Nile virus and St. Louis encephalitis virus. Positive sense ssRNA genome is packaged in the capsid, formed by the capsid protein. The outer envelope is formed by envelope (E) protein and is the protective antigen. The genome also encodes several nonstructural proteins also (NS1-5). NS1 is produced as secretory form also. Envelope protein (E) is subsequently involved in membrane fusion between virion and host late endosomes and is synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociates from the small envelope protein M and homodimerizes. Viral antigen can also be shown in tissues by indirect fluorescent antibody staining