Recombinant JEV PreM/M Protein (a.a. 6-167) [His]

Product Overview

C-terminal 6xHis tagged PreM/M Protein (Japanese encephalitis virus) (a.a. 6-167)

Nature

Recombinant

Tag/Conjugate

His

Purity

> 95%, based on SDS PAGE

Format

Liquid or Lyophilized powder

Buffer

PBS with 8M Urea

Preservative

None

Storage

Store at 2-8°C prior to reconstitution. Following reconstitution, aliquot and freeze

Introduction

The Japanese encephalitis virus (JEV) is a flavivirus and is related to the West Nile Virus. JEV is the main cause of viral encephalitis in many countries of Asia with an estimated 68 000 clinical cases every year (WHO). Although symptomatic Japanese encephalitis (JE) is rare, the case-fatality rate among those with encephalitis can be as high as 30%. Permanent neurologic or psychiatric sequelae can occur in 30%–50% of those with encephalitis. Flaviviruses are positive-stranded RNA viruses with a 9-12- kb genome that is translated as a single polyprotein and then cleaved by host and viral proteases into structural capsid (C), premembrane (prM), envelope (E) proteins and seven nonstructural proteins. Capsid binds to viral RNA to form a nucleocapsid that is enveloped by an endoplasmic reticulum-derived membrane containing E and prM. E proteins are responsible for cellular attachment and possess a hydrophobic loop that mediates fusion of viral and host membrane.The E protein is therefore the primary target of neutralizing antibodies.

Keywords

Japanese Encephalitis Virus; JEV; PreM/M