Sample
Human serum and plasma samples
Intended Use
This Kit is used to assay the level of Human Hepatitis C virus IgM in human serum and plasma samples. The Kit is For Laboratory / Research Use Only.
Contents of Kit
1. Anti-Human Hepatitis C virus IgM Coated Microtitre Plate (96 wells) – 1 no
2. Biotinylated Human Hepatitis C virus IgM Antibody – 1 ml
3. Human Hepatitis C virus IgM Standard (concentrated, 48 ng/ml ) – 0.5 ml
4. Streptavidin-HRP Conjugate – 6 ml
5. (30X) Wash Buffer – 20 ml
6. Standard Diluent– 3 ml
7. TMB Substrate A – 6 ml
8. TMB Substrate B – 6 ml
9. Stop Solution – 6 ml
10. Instruction Manual
Storage
1. All reagents should be stored at 2°C to 8°C.
2. All the reagents and wash solutions are stable until the expiration date of the kit.
3. 30 minutes prior before use, bring all components to room temperature (18-25°C). Store all the components of the kit at its appropriate storage condition after use.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.
Precision
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Detection Range
1.5 ng/ml to 24 ng/ml
Sensitivity
Limit Of Detection: It is defined as the lowest detectable concentration corresponding to a signal of Mean of '0' standard plus 2* SD. 10 replicates of '0' standards were evaluated and the LOD was found to 1 ng/ml.
General Description
The Human Hepatitis C virus IgM ELISA is sandwich enzyme-linked immunosorbent assay (ELISA) to assay the level of Human Hepatitis C virus IgM in samples.
Citations
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