Glyphosate ELISA Kit

Sample

honey

Detection Method

cELISA

Intended Use

This kit can be used in quantitative and qualitative analysis of Glyphosate residue in honey.

Contents of Kit

1. Microtiter plate with 96 wells coated with antigen
2. Standard solutions(6×2ml/bottle)
0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb
Spiking standard solution: (1 ml/bottle) 1 ppm
3. Enzyme conjugate-Antibody solution 7 ml, red cap
4. Solution A 7 ml, white cap
5. Solution B 7 ml, red cap
6. Stop solution 7 ml, yellow cap
7. 20×concentrated Wash solution 40 ml, transparent cap
8. 2×concentrated sample diluent 50 ml, transparent cap
9. 2.5xconcentrated derivatization reagent 2 ml, black cap
10. Derivatization diluent 3 ml, black cap

Storage

Storage condition: 2-8°C.
Storage period: 12 months.

Performance Characteristics

Honey: 90±20%

Precision

Variation coefficient of the ELISA kit all less than 10%.

Detection Limit

Honey: 5 ppb

Sensitivity

0.1 ppb

General Description

Glyphosate is a a new internal absorption, high efficiency and low toxicity organophosphate herbicide which is widely used all over the world. In recently decades, it has been widely used due to its good conductivity, excellent weeding effect and the glyphosate-resistant plants have been developed and used widely. The herbicide mechanism is to block the activity of 5-enolpyruvylshikimate-3-phosphonic synthase(EPSP). This synthase is present in the biosynthesis of aromatic amino acids in the green plants, therefore, glyphosate is toxic and harmful to green plants. Its main metabolite in plants, soil and water is aminomethy Iphosphonic acid(AMPA). Although it is a low-toxicity organophosphorus herbicide, its unreasonable use will also lead to some agricultural products (such as sugar cane) damage; Secondly, it will lead to excessive residues of glyphosate in plant products, which will affect the food safety of consumers, and even cause disease, poisoning and death. Meanwhile, it will also affect the international trade of related products and death. Meanwhile, it will also affect the international trade. This kit is a new product for herbicide residue detection based on ELISA technology which only costs 45 min in each operation and can considerably minimize operation errors and work intensity.