The binding studies of this antibody to various human tissues was done using ELISA and indirect immunofluorescence. This antibody also showed comparable reciprocal cross-blocking in radioimmunoassay on LAN-1 NB cells or NB glycolipid extracts. This antibody is also recommended for the study of distribution of the GD2 antigen on various types of cancer tissues. This antibodhy was also used in the development of a chimeric receptor 3G6-CD28, which provides CD28 signaling upon specific recognition of the GD2 antigen on tumor cells. Expression of GD2 expressing cells was done by flow cytometry using 3G6. This antibody was also used in anti-GD2 therapy to test the survival and metastasis after challenge with EL4 cells.
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