Free FMC63 ELISA Kit

Name: Free FMC63 ELISA Kit
SKU: DEIA-JY25016
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

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COA

SDS

Datasheet

Key features and details

Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the reactant for FMC63. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to FMC63 captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The colour developed is proportional to the amount of FMC63 in the sample or standard. Results of samples can be determined directly using the standard curve.

Sample

Plasma, Serum

Species Reactivity

Human

Detection Method

ELISA

Intended Use

This kit is designed for the quantitative detection of FMC63 in serum and plasma using ELISA methodology. For research use only (RUO). Not for diagnostic procedures.

Contents of Kit

1. Pre-coated Microplate: 1 plate 2-8°C
2. Standards: 6 vials, -20°C
3. Sample Diluent: 15 mL, 2-8°C
4. HRP-Conjugated Antibody: 10 mL, 2-8°C
5. TMB Substrate: 10 mL, 2-8°C
6. Stop Solution: 10 mL, RT
7. Wash Buffer (20X): 30 mL, RT
8. Plate Sealer: 2 sheets, RT

Storage

2-8°C

Performance Characteristics

Intra-assay and inter-assay CVs < 30%

Citations

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