FLAG-Tag(DYKDDDDK-Tag Protein) ELISA Kit

Sample

Cell lysates, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

cELISA

Intended Use

In vitro quantitative determination of FLAG-Tag concentrations in cell lysates, cell culture supernatant and other biological samples.

Contents of Kit

The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
2. Lyophilized Standard, 2vial. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C.
3. Biotin-labeled Antibody(Concentrated, 100×), 60μl. 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin Conjugate(SABC, 100×), 120μl. 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml. 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer, 20ml. 2-8°C.
7. Antibody Dilution Buffer, 10ml. 2-8°C.
8. SABC Dilution Buffer, 10ml. 2-8°C.
9. Stop Solution, 10ml. 2-8°C.
10. Wash Buffer(25×). 30ml. 2-8°C.
11. Plate Sealer, 5 pieces
12. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

15.625-1000ng/ml

Sensitivity

9.375ng/ml

General Description

The FLAG-TAG (also known as FLAG octapeptide, FLAG epitope) was the first epitope label designed for a fusion protein and the only one to be patented. The molecular weight of AGDYKDDDDK-tag (FLAG tag) is 1012Da. The polyanionic amino acids in the FLAG label are unlikely to affect the activity of the target protein. The FLAG tag enables highly specific pull-down experiments with low non-specific backgrounds. A FLAG tag can be used in several different tests that require antibody recognition. If there is no antibody for a protein, adding a FLAG tag to the protein allows the protein to be studied with antibodies against the FLAG sequence.

Standard Curve

The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.

General Notes

The FLAG-TAG (also known as FLAG octapeptide, FLAG epitope) was the first epitope label designed for a fusion protein and the only one to be patented. The molecular weight of AGDYKDDDDK-tag (FLAG tag) is 1012Da. The polyanionic amino acids in the FLAG label are unlikely to affect the activity of the target protein. The FLAG tag enables highly specific pull-down experiments with low non-specific backgrounds. A FLAG tag can be used in several different tests that require antibody recognition. If there is no antibody for a protein, adding a FLAG tag to the protein allows the protein to be studied with antibodies against the FLAG sequence.